Detection of Mycobacteria spp in cows milk using conventional methods and PCR

Tuberculosis is a common deadly infectious disease and is important zoonotic disease. The disease can transmit to human by direct contact with the infected animals, drinking unpasteurized milk and consumption of uncooked meat. Therefore the aim of the current work is to detected of these bacteria in cow's milk. Sixty eight milk samples were taken from AL-FtheliaBaghdad. Conventional and molecular methods were used for the diagnosis of the disease. That results showed that three positive cases in direct smear, seven positive by culturing on Lowenstein Jansen media and the result of conventional methods were confirmed by PCR, using sacace MTB Complex kit.